In vitro culture of rabbit bladder acellular matrix carrying rat hair follicle stem cells

doi:10.3969/j.issn.2095-4344.2013.51.019

Abstract

Abstract:

BACKGROUND: The rise of tissue engineering has opened up new ways for tissue repair and reconstruction of the urinary tract, and the bladder acellular matrix is a better alternative material for urinary tissue engineering.
OBJECTIVE: To construct the compound of hair follicle stem cells with heterologous bladder acellular scaffold, and to observe the growth of hair follicle stem cells on the scaffold.
METHODS: Bladder acellular matrix from New Zealand rabbits were prepared and detected using scanning electron microscopy and Masson staining. Passage 3 hair follicle stem cells were statically inoculated into the surface of bladder acellular matrix using secondary sedimentation method. Under inverted microscope, cell growth was observed, and cell growth curves were drawn. Cell growth on the scaffold surface was observed through histological detection and scanning electron microscope observation.
RESULTS AND CONCLUSION: Prepared bladder acellular matrix was a white translucent film with fiber mesh structure, and no residual cells were seen. Masson staining results indicated that the bladder acellular matrix had collagen structure, and no obvious residual cells. After culture for 48 hours, hair follicle stem cells grew well around the bladder acellular matrix under inverted microscope; 1 week later, hair follicle stem cells extended and adhered to the scaffold surface. These findings indicate that hair follicle stem cells have a good biocompatibility with the bladder acellular matrix through in vitro culture.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, hair follicle, stem cells, urinary bladder

CLC Number:

R318

Peng Yu, Wang Yu-jie, Li Jia, Muratrixat. In vitro culture of rabbit bladder acellular matrix carrying rat hair follicle stem cells [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8901-8906.

Figures/Tables 5

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